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Nutrients attenuate depressive effect of metabolic acidosis on muscle protein synthesis in rats
Author(s) -
Caso Giuseppe,
Garlick Barbara A,
Sasvary Dawn,
Garlick Peter J
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1044-b
Subject(s) - acidosis , medicine , endocrinology , anabolism , metabolic acidosis , catabolism , chemistry , phenylalanine , saline , metabolism , biochemistry , amino acid
We demonstrated that metabolic acidosis for 24 hours depresses muscle protein synthesis in rats. In this experiment, we investigated whether this catabolic effect of acidosis can be reversed by provision of nutrients. Two groups of rats (n=8) were made acidotic for 24 h with intragastric doses of NH 4 Cl (20 mmol/kg body weight every 12 h) and two groups (n=8) were given an equimolar amount of NaCl (Controls). One group of acidotic and one of control were infused with amino acids (20mgN/Kg) and glucose (0.2 g/Kg) for 1 h while the other two groups received saline. Muscle protein synthesis (FSR) was measured with L‐[ 2 H 5 ]phenylalanine (150 μmol/ 100g body weight, 40 MP). Blood pH was approximately 0.2–0.3 units lower in groups treated with NH 4 Cl (P<0.001). As previously shown, acidosis depressed muscle FSR in fasted rats (FSR −21% in gastrocnemius and −29% in plantaris, P<0.05). However, the depressive effect of acidosis was reduced when rats where infused with nutrients (FSR −13% in gastrocnemius, P<0.05; and −7% in plantaris, P=NS). These results suggest that the anabolic response to nutrients attenuates the inhibitory effects of acidosis on muscle protein synthesis. Supported by NIH grants DK54991 and 5‐MO1‐RR‐10710