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Total sulphur amino acid requirement of children with end‐stage renal disease
Author(s) -
Turner Justine,
Humayun Arshad,
Elango Rajavel,
Rafii Mahroukh,
Langos Veronika,
Geary Denis,
Ball Ronald O,
Pencharz Paul B
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1042-c
Subject(s) - methionine , phenylalanine , renal function , chemistry , population , medicine , cysteine , end stage renal disease , amino acid , zoology , endocrinology , biochemistry , disease , biology , environmental health , enzyme
The total sulphur amino acid (TSAA) requirement of children with end‐stage renal disease (ESRD) was determined by indicator amino acid oxidation (IAAO) method. Four children (9.8–13.7 yr) with ESRD (glomerular filtration rate = 16–31 ml/min/1.73 m 2 ) randomly received each of six dietary levels of methionine (no cysteine) (0, 5, 10, 15, 25 and 35 mg.kg −1 .d −1 ) as part of an isocaloric and isonitrogenous diet. Breath and urine samples were collected at baseline and during isotopic steady state. TSAA requirement was determined by measuring oxidation of L− [1− 13 C]‐phenylalanine to 13 CO 2 (F 13 CO 2 ). Linear regression crossover analysis identified a breakpoint at minimal F 13 CO 2 in response to methionine intakes (Fig. 1). Figure 1. 13 C Phenylalanine oxidation (Mean ± SD) in response to methionine intakes (no cysteine) These preliminary data showed that the mean and population‐safe (upper limit of 95% CI) TSAA requirements are 12.8 and 19.9 mg methionine kg −1 .d −1 respectively. This suggests that TSAA requirements (methionine only) in children with ESRD are similar to those found in healthy children (12.9 and 17.2 mg kg −1 .d −1 respectively, unpublished data). Further studies are needed to determine minimum methionine requirements of children with ESRD in the presence of excess dietary cysteine.(CIHR supported).