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Diet‐induced increases in ICAM‐1, CD11c, and CD34 in adipose tissue of male mice
Author(s) -
Brake Danett Kay,
Robker Rebecca L,
Smith E O'Brian,
Smith C. Wayne
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1038-b
Subject(s) - adipose tissue , medicine , endocrinology , adipose tissue macrophages , biology , inflammation , cd11c , white adipose tissue , biochemistry , gene , phenotype
Obesity has been linked to cardiovascular disease, hypertension, hypercholesterolemia, insulin resistance and metabolic syndrome with elevated markers of systemic inflammation. Intercellular adhesion molecule‐1 (ICAM‐1) is a transmembrane adhesion molecule involved in leukocyte migration to sites of inflammation. In human obesity, abdominal fat deposition is positively correlated with elevations in the soluble form of ICAM‐1 (sICAM‐1). An obese state has also been correlated with increased macrophage infiltration into mouse adipose tissue. Here we investigate adipose tissue production and transcriptional regulation of ICAM‐1 in a mouse model of obesity induced by a 21% milkfat, high cholesterol diet. ICAM‐1 in serum and adipose tissue was analysed by ELISA, northern blot, real‐time quantitative PCR, and flow cytometry. After 6 months on the high fat diet sICAM‐1 levels significantly correlated with body and abdominal fat pad weights. Northern blots from adipose tissue showed significantly higher levels of ICAM‐1 mRNA in males than females. After 3 weeks on the high fat diet there was an adipose tissue specific, increase in mRNA for ICAM‐1, IL‐6 and MCP‐1 in male but not female mice. Analysis of the stromal vascular fraction of male adipose tissue revealed CD11b negative cells with increased surface ICAM‐1 and CD34. We also found two populations of F4/80+, CD11b+, and ICAM‐1+ cells, one of which was positive for CD14 and CD11c and significantly increased in response to a high fat diet. These results indicate that 3 weeks of a high fat diet induce significant increases in pro‐inflammatory factors in adipose tissue of male mice that may represent links between obesity and its associated inflammatory‐like complications.