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Beneficial Effects of n‐3 Fatty Acids on Intestinal Lipid Transport in the Psammomys Obesus
Author(s) -
Spahis Schohraya,
Delvin Edgard,
Ziv Ehud,
Seidman Ernest,
Levy Emile
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1037
Subject(s) - medicine , endocrinology , insulin resistance , lipogenesis , apolipoprotein b , hyperinsulinemia , fish oil , chemistry , insulin , lipid metabolism , biology , cholesterol , fishery , fish <actinopterygii>
Small intestine plays an important role in the pathogenesis of dyslipidemia in insulin resistance and type 2 diabetes. The aim of the study was to examine the effect of n‐3 fatty acids (FA) on the various events governing intraenterocyte lipid transport in Psammomys obesus , a model of nutritionally induced metabolic syndrome. Increased dietary intake of fish oil lowered body weight and improved hyperglycemia and hyperinsulinemia. It simultaneously decreased intestinal de novo lipogenesis and lipid esterification of the major lipid classes, e.g. triglycerides (TG), phospholipids and cholesteryl esters, particularly in insulin‐resistant and diabetic animals. Accordingly, lessened activity of MGAT and DGAT was recorded. As assessed in cultured jejunal explants incubated with either [ 14 C]‐oleic acid or [ 35 S]‐methionine, fish oil feeding resulted in diminished TG‐rich lipoprotein assembly and apolipoprotein (apo) B‐48 biogenesis, respectively. The mechanisms did not involve apo B‐48 transcription nor alter the gene expression and activity of MTP. The suppressed production of apo B‐48 by n‐3 fatty acids was rather associated with intracellular proteasome‐mediated posttranslational downregulation in insulin‐resistant and diabetic animals. In vivo studies documented the ability of n‐3 FA to impede plasma TG‐rich lipoprotein output following orally gavage or blood circulation infusion of intralipid in animals injected Triton WR‐1339. Our data highlight the beneficial impact of n‐3 fatty acids on the adverse effects of the metabolic syndrome.