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De novo lipogenesis (DNL) occurs after a 3‐day eucaloric low‐fat, high‐carbohydrate (HC) solid food diet
Author(s) -
Chong Mary Foong Fong,
Bickerton Alex,
Roberts Rachel,
Karpe Fredrik,
Hodson Leanne,
Fielding Barbara,
Frayn Keith
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1022-c
The aim of the study was to find out if DNL occurs in vivo after a 3‐day HC solid food diet using a new approach, the nonisotopic linoleate dilution method (1). A randomized cross‐over study was carried out. Eight normal healthy subjects followed a eucaloric 3‐day HC diet (10% of calories as fat and 75% as carbohydrate) and a high‐fat, low‐carbohydrate (LC) diet (40% fat; 45% carbohydrate), with a six weeks washout period in between. The fatty acid composition of each diet was matched to the composition of each subject's adipose tissue (diet‐adipose) and compared with the composition of fasting very‐low‐density lipoprotein triglyceride (VLDL‐TG) after the diets. The decrease of linoleate (18:2 n‐6 ) in VLDL‐TG below the level in the diet and AT would be a measure of newly synthesized fatty acids. All the subjects showed VLDL‐TG markedly deficit in 18:2 n‐6 and enriched in palmitate (16:0) after the HC diet compared to after the LC diet (see Figure). By the linoleate dilution method, which depends on a sufficient increase in 16:0 to decrease 18:2 n‐6 , DNL was detected in half the number of subjects after the HC diet (data not shown). The linoleate dilution method is sensitive to detect and measure DNL after a 3‐day HC solid food diet. In addition, a pattern of low 18:2 n ‐6 and high newly synthesized fatty acids (16:0), which have been associated with an increased risk of cardiovascular disease, is observed (1).Percent difference between the fatty acid composition of VLDL‐TG and the diet‐adipose tissue after each diet. [Support: Food Standards Agency].