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A simple and reliable modified method for measuring total serum antioxidant levels and its application in monitoring the antioxidant status in diabetes and hypertension
Author(s) -
Thomson Martha,
Drobiova Hana,
AlQattan Khaled K.,
PeltonenShalaby Riitta,
AlSawan Shorooq M.,
Haider Amina Jassim,
AlAmin Zainab,
Ali Muslim
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1017-d
Subject(s) - trolox , antioxidant , diabetes mellitus , vitamin c , trolox equivalent antioxidant capacity , vitamin e , chemistry , vitamin , glutathione , medicine , pharmacology , endocrinology , biochemistry , polyphenol , antioxidant capacity , enzyme
Oxidative damage by free radicals has been implicated in the pathogenesis of vascular disease in diabetes and hypertension. We have determined the total antioxidant status in diabetic and hypertensive rats before and after treatment with garlic ( Allium sativum ) and ginger ( Zingiber officinale ). The total antioxidants were measured in serum by a modified procedure reported earlier by others. The reproducibility of the assay was confirmed by determining standard curves for the know antioxidants: vitamin C, trolox (a stable analog of vitamin E) and glutathione. The interassay correlation coefficients were R 2 = 0.9984, R 2 = 0.9768 and R 2 0.9967 for trolox, glutathione and vitamin C, respectively, confirming the reliability and reproducibility of the assay. This assay was then used to determine total antioxidant levels in the serum of streptozotocin‐induced diabetic rats and two‐kidney one‐clipped hypertensive rats both before and after three weeks of treatment with aqueous extracts of garlic and ginger (500 mg/kg intraperitoneally daily). The serum antioxidant levels of rats after three weeks of treatment were significantly higher (p<0.001) then the pretreatment levels in both diabetic and hypertensive rats. We conclude from our study that 1) total antioxidants can be measured by a simple, reproducible, reliable assay and 2) the total antioxidant status can be significantly improved by treatment with garlic and ginger.