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Inhibition of lipid peroxidation and stress signaling by synergistic antioxidant nutrients in human lens epithelial cells
Author(s) -
Chitchumroonchokchai Chureeporn,
Failla Mark L
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1015
Subject(s) - ascorbic acid , lipid peroxidation , vitamin c , oxidative stress , antioxidant , chemistry , biochemistry , p38 mitogen activated protein kinases , nutrient , mapk/erk pathway , signal transduction , food science , organic chemistry
Our previous study has demonstrated that individual antioxidant nutrient significantly reduced ultraviolet B light (UVB) induced lipid peroxidation and stress signaling; JNK and P38 in human lens epithelial cells (HLE) at the similar physiological concentration of individual nutrient. However, it is optimistic that LUT, α‐TC including vitamin C may work together to prevent UVB oxidative stress in HLE. The present study investigated the effect of the combination of LUT, α‐TC and ascorbic acid on lipid peroxidation and the mitogen‐activated stress signaling pathway in HLE following UVB irradiation. Pretreatment of culture with three types of nutrients composition; a) 2 umol/L LUT, 10 umol/L α‐TC and 1 mmol/L ascorbic acid, b) half concentration of composition a, (1 umol/L LUT, 5 umol/L α‐TC and 0.5 mmol/L ascorbic acid (c) one‐fourth of composition a. (0.5 umol/L LUT, 2.5 umol/L α‐TC and 0.25 mmol/L ascorbic acid). The preliminary data showed that the all compositions of antioxidant nutrient significantly decreased lipid peroxidation by 52 ± 4%. The activation of MAPK signaling (JNK and P38) will be present. (Supported by The United Nation Start Up Fund)