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CLOVAMIDE‐TYPE PHENYLPROPENOIC ACID AMIDES, N‐COUMAROYLDOPAMINE AND N‐CAFFEOYLDOPAMINE, INHIBIT PLATELET‐LEUKOCYTE INTERACTIONS VIA SUPPRESSING P‐SELECTIN EXPRESSION
Author(s) -
Park Jae B,
Schoene Norberta
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1014
Subject(s) - platelet , p selectin , chemistry , in vitro , receptor , in vivo , pharmacology , l selectin , platelet activation , microbiology and biotechnology , biochemistry , medicine , biology
N ‐Coumaroyldopamine and N ‐caffeoyldopamine are clovamide‐type phenylpropenoic acid amides found in Theobroma cacao. In this paper, N ‐coumaroyldopamine and N ‐caffeoyldopamine were investigated to determine their effects on P‐selectin expression and platelet‐leukocyte interaction in vitro and in vivo models. At the concentration of 0.05 mM, they were able to inhibit P‐selectin expression on the platelets by 33 % (P < 0.011) and 30 % (P < 0.012), respectively. The inhibition was partially blocked by beta 2‐adrenoceptor antagonists, suggesting that beta‐2 receptors are likely engaged in the inhibition. N ‐Caffeoyldopamine and N ‐coumaroyldopamine could also suppress platelet‐leukocyte interactions in blood samples by 36 % (P < 0.013) and 32 % (P < 0.011), respectively, at the same concentration (0.05 mM). The inhibition of platelet‐leukocyte interactions is likely to be via inhibiting P‐selectin expression, not PSGL‐1, because they were not able to change PSGL‐1 expression in U937 cells. In animal study, mice administrated orally with N ‐caffeoyldopamine (0.050 and 0.100 mg per 35 g body weight) also showed great reduction in the P‐selectin expression and platelet‐leukocyte interactions by 31–45 % (P < 0.011) and 34–43% (P < 0.014) respectively. These data suggest that the clovamide‐type phenylpropenoic acid amides are able to suppress platelet‐leukocyte interactions via inhibiting P‐selectin expression.