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Dietary fish oil enhances adhesion molecule and interleukin‐6 expressions in mice with gut‐derived sepsis
Author(s) -
Yeh SungLing,
Yeh ChiuLi,
Chiu WanChun,
Hou YuChen,
Chen YueHwa
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1013-c
Subject(s) - fish oil , sepsis , intercellular adhesion molecule , medicine , cell adhesion molecule , lymphocyte , interleukin , endocrinology , immunology , chemistry , biology , andrology , cell adhesion , biochemistry , cytokine , fish <actinopterygii> , cell , fishery
This study investigated the effects of dietary fish oil (FO) supplementation on plasma intracellular adhesion molecule (ICAM)‐1 levels and leukocyte integrin expressions in gut‐derived sepsis. Mice were randomly assigned to a normal group (NC), a control group, or a FO group. The NC group was fed standard chow diet. The control group was maintained for 3 weeks on medium fat (10%, w/w) diets containing soybean oil, and the FO group replaced 50% of the soybean oil by fish oil. After that, sepsis was induced by cecal ligation and puncture (CLP) in the control and FO groups. Mice in the control and FO groups were sacrificed at 0, 6, 12, and 24 h after CLP. Mice in the NC group were sacrificed when the CLP was performed. Blood and organ samples were collected for further analysis. Results showed that compared with the control group, plasma intracellular adhesion molecule‐1 levels were significantly higher in the FO group 6 h after CLP. Intra‐lymphocyte interferon‐γ expressions in the FO groups were significantly lower, whereas interleukin (IL)‐4 expressions were higher than the control group 12 and 24 h after CLP. The expressions of leukocyte integrin were significantly higher in the FO group 12 and 24 h after CLP. The FO group had higher IL‐6 levels at 12 h in the lung, at 6 and 12 h in the kidney and at 6, 12, 24 h in the intestine after CLP. These findings suggest that pretreatment with an FO‐supplemented diet enhances adhesion molecule and inflammatory cytokine expressions during sepsis. In addition, FO supplementation reduced intra‐lymphocyte interferon‐γ and enhanced IL‐4 expressions. This change may promote the Th2‐type response and suppress the cellular immune response in gut‐derived sepsis.