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Nerve Growth Factor Induces Expression and Activity of CTP:Phosphocholine Cytidylyltransferase β2 in PC12 Cells and Cultures Primary Neurons
Author(s) -
Demizieux Laurent,
Carter Jodi M,
Nelson Randy C,
Campenot Robert B,
Vance Dennis E
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a88-c
Subject(s) - phosphocholine , nerve growth factor , microbiology and biotechnology , chemistry , primary culture , biology , in vitro , biochemistry , receptor , membrane , phospholipid , phosphatidylcholine
Nerve growth factor (NGF) is essential for growth of sympathetic neurons and promotes neuronal differentiation of PC12 cells and Neurite outgrowth increases the demand for phosphatidylcholine (PC). We hypothesized that NGF increases PC synthesis in response to this demand. We, therefore, investigated the influence of NGF on CTP:phosphocholine cytidylyltransferase (CT). Cellular CT activity, CT protein, and PC levels were increased in PC12 cells during NGF‐induced differentiation suggesting that increased CT was responsible for the increase in PC. To distinguish which CT isoform is implicated, we investigated whether or not NGF increased the protein and mRNA levels of CT isoforms. Results indicate that NGF treatment increases both the amount of CTβ 2 protein and mRNA level following NGF treatment of PC12 cells. Conversely, protein amount and mRNA expression of CTα are unchanged. We have reproduced the experiment using primary cultures of sympathetic neurons. Our results indicate that NGF also increases the expression of CTβ 2 in primary neurons, confirming the direct influence of NGF on CTβ2 mRNA and protein and protein. We conclude that promotion of neurite outgrowth by NGF involves the induction of CTβ 2 expression which increases the amount CTβ 2 protein and CT activity, thereby increasing the amount of PC required to support neurite elongation.