Premium
Effect of knock down of BRH2 on DNA recombination in trypanosomes
Author(s) -
Hall Mack,
Misra Smita,
Chaudhuri Gautam
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a82-b
Subject(s) - biology , trypanosoma brucei , rad51 , homologous recombination , gene , trypanosoma cruzi , rna interference , dna repair , genetics , leishmania , dna , plasmid , microbiology and biotechnology , rna , parasite hosting , world wide web , computer science
The breast cancer susceptibility protein BRCA2 is implicated in the DNA double strand break (DSB) repair pathway through its association with Rad51. Homologs of the BRCA2 gene (BRH2) have been identified in many mammals, as well as non‐mammals. As a part of our quest to understand the DNA damage repair and recombination process in the parasitic protozoa, Leishmania and trypanosomes, we have characterized BRH2 genes from L. major, L. donovani and Trypanosoma brucei and T. cruzi. The BRH2 proteins are 1648, 1030 and 1165 amino acid residues long respectively, for T. brucei, T. cruzi and L. major. They are coded by single copy genes in these cells. T. brucei BRH2 has 13 BRC repeats, whereas Leishmania BRH2 has two and T. cruzi BRH2 has one BRC sequences. Since among these parasite cells only T. brucei has the RNAi machinery, we employed RNAi to knock down BRH2 in these cells from a doxycycline‐inducible system. We are evaluating the recombination of two transformed plasmids to generate intact kanamycin‐resistant gene in these cells. This study will contribute towards the understanding of potential BRH2‐mediated DNA recombination/repair pathway in Leishmania and Trypanosoma. Supported by NIH grants 5‐R01AI42327‐03, 2S06GM08037‐24, and 3SO6GM008037‐33S1 to GC.