Premium
Moderate Exercise Reduces Blood Vessel Density in Allogeneic Tumors
Author(s) -
Woods Jeffrey A.,
Potter Kristin Lyons
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a813
Subject(s) - h&e stain , medicine , inflammation , blood vessel , apoptosis , mitotic index , mitosis , pathology , endocrinology , andrology , chemistry , immunohistochemistry , biology , biochemistry , microbiology and biotechnology
Previously (Zielinski et al., J. Appl. Physiol. 96: 2249, 2004), we have shown that intense, prolonged exercise could delay allogeneic tumor growth and reduce intratumoral inflammation and vascularization. In this study we wanted to determine if a more moderate exercise protocol would result in similar beneficial changes. Female Balb/cByJ (H2 d ) mice (6–8wks) were subcutaneously implanted with 20 x 10 6 EL‐4 lymphoid tumor cells (H2 b ). Mice were then randomized to either a sedentary or a treadmill exercised (30 min/d, 18m/min, 5% grade) condition. In one experiment (n = 17/group), we measured tumor volumes daily to track tumor progression and regression. In a second experiment, mice in both groups were sacrificed at Days 5, 6, and 8 (n = 6/day/group) and tumors sections were stained with hematoxylin and eosin and evaluated blindly for mitotic figures, macrophage and neutrophil content, apoptotic bodies, and blood vessels. Results Unlike our previous study with prolonged, intense exercise we found no statistically significant (p=0.16) effect on the kinetics of tumor growth in response to moderate exercise (p>0.05). We did, however, find that moderate exercise significantly (p<0.05) reduced blood vessel density (~30%) and mitotic figures (~30–40%; an indicator of cell division). Unlike our previous study, we did not find an early reduction in macrophages or neutrophil content within the tumors. Conclusion Like prolonged, intense exercise, moderate exercise reduces blood vessel density and cell division. These changes, however, were not associated with any change in tumor growth kinetics.