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Characterization of the mRNA produced from the reverse activity of human BRCA2 gene promoter
Author(s) -
Bailey Charvann Khamille,
Misra Smita,
Chaudhuri Gautam
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a81-b
Subject(s) - promoter , gene , biology , gene expression , messenger rna , regulation of gene expression , microbiology and biotechnology , cancer research , genetics
Transcription of genes from divergent bidirectional promoters is proposed to be a unique mechanism of gene expression regulation in human cells. Human BRCA2 gene expression is very stringently regulated and it is mainly expressed in the dividing cells and practically absent in the resting or quiescent cells. While studying the promoter activity of the upstream sequences of human BRCA2 gene, we discovered that this promoter is 7‐9 fold more active in the reverse orientation than the forward orientation in the quiescent human breast cancer cells. The reverse promoter activity decreases dramatically in the dividing cells. The extent of this decrease varies in different breast cancer cell lines and there is no decrease in normal human breast cells. There is no ORF reported at the reverse orientation from the human BRCA2 gene. Here we report the cloning and preliminary characterization of the mRNA that is transcribed from the reverse activity of human BRCA2 gene promoter. Our data will add to the understanding of the complex regulation of BRCA2 gene expression in human breast cells. Supported by the MMC/VICC cancer partnership grant #1U54CA091408‐010003 from NCI and the DOD grant # DAMD17‐00‐1‐0341 to GC.