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Expression and Electrophysiological properties of Cystic Fibrosis Transmembrane Conductance Regulator in Rat Oviduct Epithelium
Author(s) -
Chen Minhui,
Du Jianyang,
Ruan Yechun,
Wang Fang,
Li Manhui,
Chan Hsiao Chang,
Zhou Wenliang
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a798
Subject(s) - oviduct , cystic fibrosis transmembrane conductance regulator , forskolin , reversal potential , epithelium , medicine , electrophysiology , chemistry , endocrinology , microbiology and biotechnology , apical membrane , membrane potential , ion transporter , epithelial polarity , biology , patch clamp , biophysics , cystic fibrosis , biochemistry , membrane , genetics , stimulation
Cystic fibrosis transmembrane conductance regulator (CFTR) is responsible for the cAMP‐activated anion conductance of epithelial cell including oviduct epithelium. The aim of our present study was to investigate the electrophysiological properties of CFTR in rat oviduct epithelium. Under whole‐cell patch‐clamp condition, oviduct cells responded to intracellular cAMP (100μM) with a rise in inward current in Gap‐free mode. The cAMP‐activated current exhibited a linear I–V relationship and time‐ and voltage‐independent characteristics. Cells were held at −70mV and then stepped from −120 to +120 mV in 10‐mV increments. The current was inhibited by the Cl − channel blocker diphenylamine 2, 2′‐dicarboxylic acid (DPC) in a voltage‐dependent manner. The reversal potentials of the cAMP‐activated currents in symmetrical Cl − solutions were close to the Cl − equilibrium, 0mV. In short‐circuit current measurement in cultured rat oviduct epithelial cells, the Isc was increased by an adenylate cyclase activator forskolin (10μM), which was added to basolateral membrane, and then inhibited by apical addition of DPC (1mM). These electrophysiological properties of the cAMP‐activated Cl − conductance in the oviduct were consistent with those reported for CFTR. In support of the functional studies, reverse transcription polymerase chain reaction also revealed the presence of CFTR mRNA in cultured oviduct epithelium. CFTR may play a role in modulating fluid transport in the oviduct.