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Transcriptional Regulation of 19S lid and COP9 signalosome in Schistosoma mansoni
Author(s) -
Cabral Fernanda Janku,
Sa Renata G.,
Macedo Elenice,
Brigato Olinda,
Rodrigues Vanderlei
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a79-b
Subject(s) - cop9 signalosome , biology , schistosoma mansoni , proteasome , parasite hosting , microbiology and biotechnology , immunology , helminths , protease , schistosomiasis , biochemistry , peptide hydrolases , enzyme , world wide web , computer science
The trematode Schistosoma mansoni causes intestinal schistosomiasis. It has a complex developmental plan including six stages of life and experiencing two kinds of hosts an invertebrate and a vertebrate. Our objective is to understand transcriptional regulation of components of proteasome 19S and Comorphogenesis 9 ‐ COP9 signalosome (CSN) through parasite development. CSN is an evolutionary conserved protein complex that resembles the lid subcomplex of proteasomes. Using S.mansoni databases we searched for sequences CSN 1 to 8 like and the subunits of lid and base of PA700. RT‐ PCR was performed using specific primers and total RNAs from adult worms, cercariae and eggs to evaluate the levels of lid and COP9 transcripts. Northern Blot for adults and cercariae were performed as well. Our results evidence that both lid and COP9 are conserved in this parasite, and expression of the RPN1 and RPN9 is 20 times more abundant in adults than cercariae. In contrast of proteasome lid subunits, CSN3 and CSN7 are 3 times more abundant in cercariae than adults, suggesting a downregulation of lid, thus modulating proteasome activity, which would explain the low proteolysis rate in cercariae. It may suggest that COP9 may play a role as an alternative lid for proteasome 26S in developmental stages of parasite. Pull‐down assays using other parasite forms in cercariae‐adults transition would confirm this statement. Future prospects towards investigation of this pathway will clarify some aspects of parasite’s development. FAPESP, CNPq, USP