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gene bookmarking mechanism
Author(s) -
Yang Jianing
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a79
Subject(s) - bookmarking , protein phosphatase 2 , mitosis , transcription factor , condensin , biology , gene , microbiology and biotechnology , genetics , chromatin , protein subunit , cohesin
‐gene bookmarking mechanism A transcription factor called HSF2 plays a critical role in mediating hsp70i gene bookmarking by binding to hsp70i promoter, recruiting protein phosphatase 2A (PP2A), and interacting with condensin enzyme by binding to the CAP‐G subunit to allow the PP2A to dephosphorylate and inactivate this condensin, thus preventing compaction of this region of chromosomal DNA. [Xing etc. Science 307, 421 (2005)] It is critical for the cell to be able to express the hsp70i gene in G1 phase for cell stress survival. In this study, our focus is to further understand this gene bookmarking mechanism by investigating two hypotheses: whether mitotic‐dependent SUMO modification of HSF2 regulates its interaction with PP2A, and whether HSF2 interaction with PP2A regulates its phosphatase activity. We predict that the mitotic‐dependent sumoylation is responsible for mediating the interaction between HSF2 and PP2A in the mitotic stage of the cell cycle and that PP2A phosphatase activity will increase upon binding with HSF2. In vitro binding experiments show that PP2A can bind with HSF2 specifically. Experiments are in progress to test the hypotheses described above. The research is supported by NIH.