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Protective effects of total flavones extracted from chrysanthemum morifolium on rat brain against cerebral ischemia/reperfusion injury
Author(s) -
Lin Lin,
Ma Xin,
Wu Liping,
Shen Lei,
Gong Jieqin,
Shen Jun,
Jiang Huidi,
Xia Qiang
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a779-b
Subject(s) - malondialdehyde , superoxide dismutase , medicine , reperfusion injury , tetrazolium chloride , ischemia , anesthesia , neuroprotection , pharmacology , oxidative stress
The aim of this study was to investigate the protective effect of total flavones extracted from chrysanthemum morifolium (TFCM) on rat brain against cerebral ischemia/reperfusion (I/R) injury and determine the possible mechanism. Adult male Sprague‐Dawley rats were subjected to middle cerebral artery occlusion for 90 min followed by reperfusion for 22 h. The infarct area of brain was assessed in brain slices stained with 2% 2,3,5‐triphenyl tetrazolium chloride. Spectrophotometric assay was used to determine the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in brain tissue. Neurological deficit was evaluated by using a 10‐point neurological function scoring system. Administration of TFCM at 50, 100 or 200 mg/kg produced 23.1%, 25.6% or 59.4% reduction in neurological score respectively ( P <0.05), and reduced the percentage of infarction in the ipsilateral hemisphere by 27.54%, 38.45% or 47.82% ( P <0.01) compared with the vehicle group. At the same time, treatment with TFCM at 200 mg/kg significantly attenuated the decrease in SOD activity and the elevation of MDA content (p<0.05 vs. vehicle group) in the ipsilateral hemisphere. The results indicate that treatment with TFCM provide significant protection against cerebral I/R injury in rats, in which the antioxidant action of TFCM may be involved. (Supported by ZJTCMB grant G20010358)