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Histone Deacetylase 1 Is a Positive Effect in Expression of p21 in p53‐independent Cells
Author(s) -
Hung JanJong,
Tsai HuiLing,
Chang WenChang
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a77-a
Subject(s) - histone deacetylase , cancer research , histone deacetylase inhibitor , histone deacetylase 2 , histone deacetylase 5 , hdac11 , chemistry , histone , biology , gene , biochemistry
The previous studies indicate that Tricostatin A (TSA) is a stimulator of p21. In our study, we found that HDAC1 has the different effect in expression of p21 in p53‐dependent and ‐independent cells. The experiments of HDAC1 overexpression and that of HDAC1 knockdown in p53‐dependent and ¡Vindependent cells revealed that HDAC1 was negative effect in the expression in p53‐dependent cells, but was positive effect in p53‐independent cells. Furthermore, we studied the mechanism and found that the recruitment of p300 to Sp1 binding region of the p21 promoter was increased obviously in p53‐null cells under the overexpression of HDAC1, but that was not observed in p53‐dependent cells. At last, overexpression of Sp1(K703/A) which can not be acetylated could increase the transcriptional activity of p21 in p53‐null cells, but not in p53‐dependent cells. Taken together, HDAC1 has the different effect to the p21 in the absence or presence of p53.