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Characterization of the angiotensin II (AngII) model of hypertension for inter‐laboratory investigation±
Author(s) -
Osborn John W.,
Fink G.,
Sved A.,
Toney G.,
Raizada M.,
King Andrew,
Guzman Pilar,
Sved J.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a755
Subject(s) - angiotensin ii , endocrinology , medicine , chemistry , blood pressure , angiotensin 1 , renin–angiotensin system
A recent report showed that in a well established model of salt‐dependent hypertension—the AngII‐induced model—arterial pressure (AP) did not increase in when AngII was given intravenously (iv) by osmotic minipumps and AP was measured by telemetry in rats on a normal salt diet (Hypertension 42: 798, 2003). To address this controversy we assessed the AP responses to AngII in rats on three levels of dietary salt (0.1%, 0.4% and 2.0% NaCl) in one laboratory, and determined the reproducibility of the model in four separate laboratories. AP was measured by telemetry for 7 control days and 14 days of AngII by osmotic minipump. Initial studies showed that minipumps were unreliable for iv AngII; therefore AngII was given subcutaneously (150 ng/kg/min). Control AP was similar between groups but the response to AngII was directly related to salt intake. By Day 14 of AngII AP increased 10 + 3 (n=6), 27 + 7 (n=6) and 44 + 9 (n=6) mmHg in 0.1%, 0.4% and 2.0% NaCl rats respectively in one laboratory. Moreover, the responses to AngII in rats consuming 0.4% and 2.0% NaCl diets were similar in 4 laboratories. By Day 14 of AngII AP increased 17 + 3 (n=27) and 33 + 4 mm Hg (n=26) for 0.4% and 2.0% NaCl rats respectively. We conclude that the AngII‐induced model is salt‐sensitive and is reproducible among different laboratories when AngII is infused subcutaneously by osmotic minipumps. NIH Grant HL 076312