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Nucleosome assembly protein plays a critical role in the pro‐proliferative phenotype observed in pulmonary microvascular endothelial cells.
Author(s) -
Clark Jennifer Ellen,
Stevens Troy
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a749-c
Subject(s) - transfection , microbiology and biotechnology , biology , nap , population , doubling time , cell culture , cell growth , cell cycle , phenotype , downregulation and upregulation , cell , cancer research , immunology , medicine , genetics , gene , environmental health , neuroscience
Nucleosome assembly protein (NAP) has a role in inducing a pro‐proliferative phenotype in all cells during development and several cell types in the adult including hematopoetic precursors. Pulmonary microvascular endothelial cells (PMVECs) proliferate rapidly when isolated and grown in culture compared to pulmonary artery endothelial cells (PAECs). mRNA analysis revealed PMVECs produce 3.5 fold greater NAP than PAECs. We hypothesize the PMVEC pro‐proliferative phenotype is maintained by NAP. NAP overexpressing PAECs using retroviral transfection revealed induction of a proliferative profile similar to that observed in PMVECs by growth curves and cell cycle analysis. PMVECs proliferate faster than PAECs in culture, demonstrating a population doubling time of 36 hours compared to 55 hours for PAECs during log phase growth. PAECs transfected with a retrovirus for the overexpression of NAP displayed a growth curve similar to PMVECs and having a population doubling time of 47 hours during log phase growth. Serum restricted cell cycle synchronized cultures were analyzed daily for S phase percentages following exposure to 10% FBS DMEM. During log phase growth, the percentage of cells in S phase from the PMVEC population was greater than that observed for PAECs during log phase growth. In contrast to wild type PAECs, PAECs overexpressing NAP displayed an increase in S phase percentage during log phase growth. NAP plays a critical role in the pro‐proliferative phenotype of PMVECs. Supported by HL 066299, HL 060024.