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Effect of temperature on elastase and collagenase response in lung interstitial hydraulic conductivity
Author(s) -
LaiFook Stephen J.,
Houtz Pamela K.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a744-a
Subject(s) - elastase , collagenase , chemistry , albumin , diffusion , flow (mathematics) , lung , medicine , biochemistry , enzyme , physics , geometry , mathematics , thermodynamics
Rabbit lungs were inflated with silicon rubber via airways and vessels (J. Appl. Physiol. 97:2139, 2004). The lungs were cut into 1 cm thick sections. Two chambers were bonded to each section to enclose the interstitium surrounding an arterial segment. Driving pressure for flow between the chambers was 5 cmH 2 0. The flow of 5 g/dl albumin‐ringer solution was measured at 22 °C then at 37 °C. No increase in flow occurred (flow ratios, 1.14 ± 0.20 (SD), n = 7; P > 0.05). The flow of 5 units/dl leukocyte elastase in 5 g/dl albumin‐ringer solution was measured at 22 °C and then at 37 °C. In 4 of 7 segments, elastase produced a 90% increase in flow at 22 °C (flow ratios, 1.9 ± 0.94) but no further increase at 37 °C (flow ratios, 1.0 ± 0.83). A similar result was obtained in 3 segments in which elastase caused a reduced flow. In 9 segments, collagenase (0.027 g/dl) increased flow by 80% (flow ratios, 1.8 ± 0.66), with no further increase at 37 °C (flow ratios, 1.2 ± 0.52). The insignificant 14% and 20% increased flows with temperature were consistent with the 20% reduced viscosity at 37 °C. Results indicated that interstitial degradation by elastase and collagenase was diffusion limited. Supported by NIH grants HL 36597 and HL 40362