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Changes in Brain ATP during Hypoxia
Author(s) -
Kung David K.,
Beylin Alexander,
Miekisiak Grzegorz,
Dale Nicholas E.,
Winn H.R.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a734
Subject(s) - cerebral blood flow , hypoxia (environmental) , chemistry , medicine , anesthesia , endocrinology , oxygen , organic chemistry
The acute temporal changes in brain adenine nucleotides during acute hypoxia are unclear. Consequently, we measured changes in cerebral blood flow (CBF) and extra‐cellular brain ATP concentration in frontal cortex during 15 sec of hypoxia (10% O 2 ) in anesthetized, ventilated, temperature stabilized rats with cranial windows (dura intact). CBF was measured by laser Doppler and ATP on‐line by microelectrode biosensors. RESULTS Hypoxia of 15 sec (PaO 2 = 38.3± 6.6 mmHg SD) resulted in an increase in CBF (62.7% increase ± 24.4% SD) which lasted 53.7 ± 13.5 sec (n=14). ATP decreased during hypoxia and returned toward base‐line values before CBF regained pre‐hypoxic levels. The decrease in ATP preceded the onset of increase in CBF by 3.19 ± 2.05 sec. The nadir in ATP was achieved 8.35 ± 0.74 sec after the peak of the CBF. The figure illustrates a typical response to 15 sec of hypoxia. CONCLUSIONS Extra‐cellular ATP concentrations in frontal cortex during transient systemic hypoxia: 1.decrease before the rise in CBF; 2. return toward baseline before CBF [supported by R01(NS 21076 NINCDS) to HRW]