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Increased body fat results in attenuated endothelium‐dependent dilation of rat aorta
Author(s) -
Chitaley Kanchan,
Pham Matilda,
Luttrell Ian,
Morton Greg,
Ogimoto Kayoko,
Wessells Hunter,
Kim Francis
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a727-b
Subject(s) - myograph , endocrinology , medicine , sodium nitroprusside , chemistry , vasodilation , endothelium , aorta , obesity , body weight , endothelial dysfunction , nitric oxide
Obesity is associated with high cardiovascular risk. We tested the hypothesis that obesity, independent of dietary fat content, results in vascular endothelial dysfunction. Rats were fed a high fat (HF; kcal: protein 20%; carbohydrate 20%; fat 60%), low fat (LF; kcal: 20% protein; 70% carbohydrate; 10% fat), or normal chow diet (equal to LF, but not enhanced for better taste). At 8wks of HF or LF diet, % body fat and weight were measured, and aortas excised for the assessment of acetylcholine (Ach) and sodium nitroprusside (SNP)‐induced endothelium‐dependent or independent dilation (respectively) using a wire myograph. Percent body fat (20±1.3 vs 23±1.3%, n=10) and body weight (568±12 vs. 610±17g, n=10) between LF and HF diet‐fed rats, respectively, was not significantly different, possibly due to high food consumption in both groups. However, % body fat (5.11, n=2) and weight (441±27g, n=5, p<0.05) were lower in chow‐fed rats compared to both LF and HF groups. Relaxation to Ach was not different in aortas from HF and LF rats (max % relaxation: 51±5 vs 56 ±9, respectively n=4 each), however, maximum relaxation to Ach was significantly increased in aorta from chow (76±5% n=4, p<0.05) compared to LF‐diet rats. Relaxation to SNP was not different between groups. These data suggest that increased % body fat and weight result in attenuated endothelium‐dependent vasodilation regardless of the dietary fat content. NIH: HL04346