Premium
Age‐related impaired endothelium‐dependent dilation is associated with increased vascular endothelial cell protein expression of NF‐kB in humans
Author(s) -
Eskurza Iratxe,
Donato Anthony J,
Kahn Zachary D,
Silver Annemarie E,
Seals Douglas R
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a721-d
Subject(s) - endothelial dysfunction , brachial artery , endothelium , inflammation , medicine , endothelial stem cell , vascular endothelial growth factor b , vascular remodelling in the embryo , endocrinology , pathophysiology , immunology , vascular endothelial growth factor , biology , vascular endothelial growth factor a , vegf receptors , blood pressure , biochemistry , in vitro
Chronic vascular inflammation is involved in all stages atherosclerosis including endothelial dysfunction. Age, a major atherogenic risk factor, is considered a state of systemic chronic inflammation and associated with impaired endothelium dependent‐dilation (EDD), the central feature of endothelial dysfunction. The main aims of this study were to determine if: 1) vascular (venous) endothelial cell protein expression (ECPE) of NF‐κB (quantitative immunofluorescence), a major redox‐sensitive transcription factor that mediates expression of inflammatory mediators, is increased with aging; and 2) the age‐related decline in EDD is associated with increased endothelial NF‐κB. Endothelial cell protein expression of NF‐κB was 52 % greater in older (n=11, 64±2 years, MEAN±SE) compared with young (n=8, 24±1 years) subjects (0.21±0.02 vs. 0.44±0.07 ECPE/HUVEC; P<0.001). Brachial artery flow‐mediated dilation (FMD), a measure of EDD, was 60 % lower in older than young subjects (3.1±0.6 vs. 7.6±0.8 %, P<0.001), whereas endothelium independent dilation (EID) was similar in both groups. Brachial artery FMD was inversely related to NF‐κB in the pool sample (r=−0.70, P=0.008), but not to EID. These preliminary results indicate that endothelial NF‐κB protein expression in increased with age and related to impaired EDD in healthy humans. Supported by NIH AG006537, AG013038, and AG022241.