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A novel protein RGPR‐p117 enhances regucalcin gene expression in kidney NRK52E cells: The role as nuclear transcription factor
Author(s) -
Yamaguchi Masayoshi
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a71-b
Subject(s) - luciferase , transfection , transcription factor , chemistry , gene , microbiology and biotechnology , gene expression , mutant , reporter gene , biology , biochemistry
Regucalcin has been demonstrated to play a multifunctional role as regulatory protein in the intracellular signaling system of many cell types [Yamaguchi, Int J Mol Med 15:371‐389, 2005]. RGPR‐p117 was discovered as a regucalcin gene promotoer region‐related protein that binds to the TTGGC sequence in the promoter region of the rat regucalcin gene using a yeast one‐hybrid system [Misawa and Yamaguchi, Int J Mol Med 8:513‐520, 2001]. Whether RGPR‐p117 is involved in the regulation of gene expression using HA‐RGPR‐p117/phCMV2‐transfected NRK52E cells was investigated. Immunocytochemical observation showed that HA‐RGPR‐p117 was localized in the nucleus of HA‐RGPR‐p117/phCMV2‐transfected cells. Overexpression of RGPR‐p117 enhanced the expression of regucalcin mRNA and its protein level in the transfected cells. Overexpression of RGPR‐p117 also enhanced luciferase activity in the luciferase reporter assay system with construct of the TTGGC sequence. This enhancement was not observed in the mutant with deletion of the TTGGC sequence. The effect of RGPR‐p117 on the luciferase activity was shown to relate to its phosphorylation using various signaling inhibitors. This study demonstrates that RGPR‐p117 plays the role as nuclear transcription factor for the regucalcin gene expression that is involved in the TTGGC sequence.