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Acute stretch enhances expression of Akt and VEGF in healing mouse skin
Author(s) -
Shrader Carl,
Prescott Mary,
Luo Jia,
Cilento Eugene,
Reilly Frank
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a709
Subject(s) - protein kinase b , proliferating cell nuclear antigen , angiogenesis , vascular endothelial growth factor , wound healing , western blot , medicine , downregulation and upregulation , blot , growth factor , vascular endothelial growth factor a , chemistry , pathology , cancer research , microbiology and biotechnology , immunology , biology , signal transduction , vegf receptors , receptor , immunohistochemistry , biochemistry , gene
Western blot analysis identified two mitogenic intermediates of insulin signaling that are induced by rapid intermittent stretching of mouse skin. This study was a follow up to ones suggesting that tissue insulin‐1 and proliferating cellular nuclear antigen (PCNA) are stretch responsive. Flaps of skin were stretched for 3 min using linear plus hemispherical load cycling with a skin hook and an inflated subcutaneous silicone catheter. Unstretched skin served as postoperative controls. Western blots revealed that protein kinase B (Akt) and vascular endothelial growth factor (VEGF) are specifically induced by acute stretch. Results reconfirmed upregulation of tissue insulin‐1 and PCNA as reported previously. Expression was optimal by 24h for VEGF and by 4d for Akt. These data demonstrated that Akt and VEGF are acute stretch responsive. Since Akt and VEGF are strongly implicated in angiogenesis and cell growth, our findings suggested that long‐term skin flap viability is enhanced by induction of beneficial stretch‐responsive mitogens promoting wound healing.