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Improving Rana pipiens health status maintains capillary hydraulic conductivity (Lp) response to a change in shear stress (ΔSS)
Author(s) -
Flood Mary H.,
Canine Curt R.,
WipkeTevis Deidre D.,
Williams Donna A.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a707-c
Subject(s) - rana , zoology , medicine , chemistry , endocrinology , biology
Infection may be a confounding factor in studies of individual capillaries in situ . Recently, we tested an anti‐parasitic in combination with the standard antibiotic to improve frog health status. Since the relationship between ΔSS and L p appears to be a sensitive index of healthy capillary function, the purpose of the present study was to test capillary responses to ΔSS in frogs treated with our standard care, gentamicin (G, 1.3mg·L −1 ; n=16) versus G plus levamisole HCl (L, 12mg.L −1 ; n=18). We hypothesized that capillaries would retain their ability to adjust L p after a ΔSS with G/L treatment. Frog mesenteric true capillaries, without rolling or sticking WBC, were cannulated individually. L p was assessed on the first occlusion using the modified Landis technique. Mean L p was 6.7±1.5 x 10 −7 for G and 3.5±0.4 x 10 −7 cm.s −1 .cmH 2 O −1 for G/L ( P =0.05). Regression analysis revealed more outlier values for L p in G‐ (38%) versus G/L‐treated (22%) frogs. A significant relationship between ΔSS and L p existed for both groups with the slopes indistinguishable (G, 0.04±0.02, R 2 =0.49 vs G/L, 0.07±0.02, R 2 =0.44; P <0.04). Fewer outliers suggest that treatment with G/L improves the success rate of experiments by decreasing parasites as well as bacteria in the frog. The ability of individual capillaries to adjust to a range of changes in shear stress may be one characteristic of healthy barrier function. Improving the health status of Rana pipiens with G/L did not diminish capillary functional adjustment to ΔSS stimuli. Supported by NIH RO1 HL63125.