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Partial cloning of the coding sequence of ERK2 gene in sheep uterine artery
Author(s) -
Zhang Haitao,
Zhang Lubo
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a696-b
Subject(s) - cloning (programming) , coding region , gene , sequence (biology) , coding (social sciences) , biology , computational biology , genetics , computer science , mathematics , programming language , statistics
Our recent studies demonstrated that extracellular signal‐regulated kinase (ERK) played an important role in the regulation of vascular contractility of ovine uterine arteries. In addition, pregnancy significantly increased ERK protein and mRNA levels in the uterine artery. In an effort of attempting to study the regulation of ERK2 gene expression in the uterine artery, we designed primers based on known ERK2 mRNA sequences from human, rat, mouse and bovine. A 539 bp segment of ERK2 gene coding sequence was successfully amplified in the sheep uterine artery by RT‐PCR. Homology analysis revealed that nucleotide sequence of the segments between sheep and human are 94% identical, which translate into 2 amino acids alteration, and between sheep and cow, 97% DNA sequence are identical, which also translate into 2 differences in amino acids sequence, suggesting that the ERK2 gene is highly conserved among species, reflex its important regulatory function in cellular processes.