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Single nucleotide polymorphisms (SNPs) of trace amine receptors
Author(s) -
Tang ChihMin,
Siecke Neil W.,
O’Connor Daniel T.,
Insel Paul A.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a692-d
Subject(s) - single nucleotide polymorphism , snp , receptor , allele , tyramine , tryptamine , biology , chemistry , microbiology and biotechnology , genetics , genotype , biochemistry , gene
Certain amines (tyramine, β‐phenylethylamine, tryptamine, and octopamine) are found in trace amounts in peripheral tissues and the nervous system. Trace amine receptors (TARs) belong to the superfamily of G protein‐coupled receptors and their signal transduction has been linked to cardiac complications. Here, we assessed for SNPs in the coding region of both TAR1 and TAR3 from 52 subjects [28 normotensive (NTs)/ 24 hypertensive (HT), 42 male/10 female, 29 Caucasian/23 African American, mean age 47±2]. We identified three TAR1 SNPs only in HTs: CGT‐>TGT, Arg 25 ‐>Cys (2 HTs), CGC‐>TGC, Arg 123 ‐>Cys (1 HT), and TGC‐>TGT, Cys 267 ‐>Cys (4 HTs); while two TAR1 SNPs, ACA‐>ACC, Thr 39 ‐>Thr (1 NT) and AGA‐>AGG, Arg 314 ‐>Arg (1 NT), were found only in NTs. The sixth SNP of TAR1, GTA‐>GTG, Val 290 ‐>Val, was detected in 6 NTs and 2 HTs. We also identified several SNPs in TAR3: AAC‐>AAT, Asn 25 ‐>Asn (4 NTs and 4 HTs), TTT‐>TAT, Phe 69 ‐>Tyr (1 HT), TCC‐>TCT, Ser 114 ‐>Ser (2 NTs and 3 HTs), GCA‐>ACA, Ala 278 ‐>Thr (1 NT and 2 HTs), and TCG‐>TTG, Ser 335 ‐>Leu (1 NT and 2 HTs). The only SNP with high allelic frequency (22.1%) in this study was a premature stop codon in TAR3, AAA‐>TAA, Lys 61 ‐>stop, found in 13 NTs and 6 HTs. These results indicate that although both TARs are polymorphic, most SNPs, other than TAR3 AAA ‐>TAA, are not common. Larger sample size is needed to confirm and extend these findings but the data suggest that there are differences in expression of TAR1 and TAR3 SNPs between normotensive and hypertensive subjects. (Supported by grants from NIH).