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MAC‐1 expression on human neutrophils is enhanced by cytochalasin B through a mechanisms depending on multiple intracellular signaling pathways
Author(s) -
Liao ChangHui,
Wang MayYing
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a690-b
Subject(s) - wortmannin , bapta , microbiology and biotechnology , calcium in biology , phosphorylation , chemistry , intracellular , mapk/erk pathway , cytochalasin d , p38 mitogen activated protein kinases , signal transduction , protein kinase b , biology , biochemistry , cell , cytoskeleton
fMLP induced CD11b expression on human neutrophil. Cytochalasin B (CB), with a concentration dependent manner, potential increase CD11b expression on fMLP stimulated human neutrophils. CD11b expression by CB and fMLP was inhibited by pretreatment of neutrophils with BAPTA/AM, PD98058, wortmannin, SB203580, and genestin. Furthermore, CB enhanced fMLP‐induced tyrosine phosphorylation in human neutrophils and markedly enhanced the phosphorylation of mitogen‐activated protein kinase (ERK), P38 kinase, and PI3 kinasee caused by fMLP. Wortmannin inhibited CB enhanced fMLP‐induced intracellular calcium mobilization, indicating that PI3 kinase modulate intracellular calcium mobilization. PD98059, SB203580 did not inhibit CB enhanced fMLP induced intracellular calcium mobilization, indicating that ERK and P38 could be the down stream of calcium. BAPTA/AM inhibited the ERK activation support this idea. Furthermore, PD98059, SB203580, BAPTA/AM did not affect the Akt phosphorylation, this data shown that PI3K/Akt was an up stream signaling. CB did not manipulate the fMLP receptor on human neutrophil. This data shown that CB potential the effect of fMLP was not due to increase in the number of fMLP receptor. According to these information, CB enhcanced fMLP‐induced CD11b expression through activation of PI3 kinase followed by intracellular calcium, CB also activate P38 which is independent of PI3 kinase.