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Evaluation of Respiratory Pharmacology in the Conscious Rat using Whole Body Bias Flow Plethysmography.
Author(s) -
McHugh Nansie A,
Miyamoto Mutsumi,
Kelly Catherine M,
Karantabias George C,
Gosselin Sylvie J
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a674-b
Subject(s) - plethysmograph , respiratory system , anesthesia , medicine , expiration , tidal volume , muscle relaxant , lung volumes , respiratory rate , pharmacology , lung , blood pressure , heart rate
The non‐invasive measurement of respiratory function in conscious rats is a necessary component of Safety Pharmacology assessment. We routinely measure respiratory rate, tidal volume and peak inspiratory and expiratory flow in the conscious rat using a whole body plethysmograph by determining lung capacity. Thoracic cavity movement is converted by pressure transducers into a digital signal that is recorded and analyzed by BioSystem XA software (Version 2.9). The purpose of this study is to test the measurement of a wide range of possible respiratory values by recording measurements following administration of various compounds known to illicit significant respiratory responses. Morphine Sulfate, an opioid (10 and 20mg/kg, iv; and 100mg/kg, po) and Baclofen, a skeletal muscle relaxant (10, 20, and 30 mg/kg, po) were used as respiratory depressants. Theophylline (10 and 20 mg/kg, iv) was chosen based on its stimulant effects on respiratory parameters. Here we validate the system and test several compounds with known respiratory effects as potential standard controls in future studies.