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Toxicogenomics of Single Wall Carbon Nanotubes
Author(s) -
Clement Jade Q.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a67-b
Subject(s) - toxicogenomics , nanomedicine , nanotechnology , carbon nanotube , computational biology , microarray , human health , chemistry , gene , biology , gene expression , materials science , medicine , nanoparticle , biochemistry , environmental health
For realization of the full potential of nanotechnology and nanomedicine, it is important to clarify the exact nature of nanomaterials and their interaction with the living world as well as finding counter measures for their adverse effects on living systems. A thorough understanding of the hazardous impact of carbon nanomaterials on human health will not only provide a scientific base for formulating safe‐guard standards to protect susceptible populations, but also enhance the safety confidence in nanotech development. The main goal of the present research is to delineate the effect and molecular mechanisms of single wall carbon nanotubes (SWCNTs) on human cells and their exposure‐specific gene expression profiles. Cellular and molecular techniques including DNA microarray for global cellular gene activity analysis, RT‐PCR, Northern blotting, and cytotoxicity assays such as cell viability and growth rate were used to characterize the biological and toxicological effects of SWCNTs. Comprehensive toxicological profiles were obtained through fluorescently labeled cRNA oligo microarray and chemiluminescently labeled cDNA array technology. Results suggest that SWCNTs may preferentially affect oncogenes and tumor suppressor genes as well as genes for cell surface receptors. The application of different array platforms as a means of high throughput toxicogenomic data validation is also discussed. The author wishes to acknowledge the support of the Texas Institute for Intelligent Bio‐Nano Materials and Structures for Aerospace Vehicles, funded by NASA Cooperative Agreement No. NCC‐1‐02038