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Effects of pioglitazone on isolated human endothelial progenitor cells
Author(s) -
Redondo Santiago,
Hristov Mihail,
Tejerina Teresa,
Weber Christian
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a668-d
Subject(s) - pioglitazone , progenitor cell , endothelial progenitor cell , fibronectin , peripheral blood mononuclear cell , chemistry , flow cytometry , andrology , microbiology and biotechnology , medicine , endocrinology , biology , in vitro , stem cell , cell , biochemistry , diabetes mellitus , type 2 diabetes
Objective We aimed to study the effects of the thiazolidinedione pioglitazone on human endothelial progenitor cells (EPCs) in vitro. Methods EPCs were obtained from the mononuclear cells of human buffy coats. Characterization was performed on day 7 by flow cytometry analysis for DiLDL/Lectin, CD14, CD31 and kinase‐insert domain receptor (KDR). Formation of colony forming units (CFUs), as well as adhesion on fibronectin matrix under laminar shear flow was also assessed. Results Pioglitazone, at a concentration of 1μM and 72h of incubation, increased the EPC number (155.3±17.9% vs. 101.2±3.0% in the control without treatment). It also augmented the number of EPC‐CFUs (149±3.2% vs. 100.0±6.9%). The most dramatic phenotypic change was achieved after analysing expression of KDR. Pioglitazone increased the total number of KDR + EPC from 13.3±0.4% to 39.4±4.5%. In addition, this treatment significantly increased the EPC adhesion on fibronectin under laminar shear flow from 100.0±13.9% to 173.1±26.7%. However, these effects were not achieved after incubation with pioglitazone at the higher concentartion of 10μM. Conclusions Pioglitazone was able to exert a stimulatory effect on cultured human EPCs in a biphasic manner (1μM but not 10μM), as this has been revealed by increasing EPC number and function, and improving the EPC adhesion under flow conditions.