Platelet Microparticle Formation is Increased in Mice with Diet‐induced Diabetes

Type 2 diabetes is associated with increased platelet microparticle (PMP) formation and platelet P‐selectin expression which contribute to a prothrombotic state. The purpose of this study was to determine if PMP formation and P‐selectin expression were increased in the high‐fat diet induced mouse model of diabetes (DID). C57BL6/J male mice, age 4 weeks, were randomized to standard or 60% beef lard chow for 4 months. One mL of sodium citrated blood was withdrawn from isofluorane anesthetized mice (N=5/group). Samples were incubated with PBS or A23187 for 10 minutes. Samples were then incubated with PE‐conjugated anti‐mouse CD61, FITC‐conjugated anti‐mouse CD62P or isotype control, fixed with 1% paraformaldehyde, and placed on ice until flow cytometry acquisition and analysis. After 4 months of diet all DID mice were obese (34.7±0.9 v.50.0±1.2 gm) and hyperglycemic (181.0±13.1 v. 235.2±10.4 mg/dl). We found that PMP formation was elevated in the DID mice compared to control (2569±324 v. 4802±791/10000 platelets, p=0.049) and when stimulated (3155±187 v. 5487±679/10000 platelets, p=0.016). There was no significant difference in P‐selectin expression between DID and control mice (13.4±0.3v. 13.6±.0.4, mean channel fluorescence (MCF), p=0.713) or when stimulated (24.4±2.4 v. 23.0±1.3 MCF, p=617). These findings are similar to other studies in which diabetes is associated with increased platelet microparticle formation and may indicate a prothrombotic state in the diet induced model of diabetes. Supported by NIH 1 F31 NR009318‐01, NIH NINR RO1 05028, and NIH HLB 58859