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Coagulation Profiling of Human, Non‐human Primate, Pig, Dog, Rabbit, and Rat Plasma: Pharmacologic Implications
Author(s) -
Neville Brian,
Fareed J,
FlorianKujawski M,
Cera L,
Duff Rick,
Valero Antonio,
Beusing R,
Hoppensteadt Debra,
Kennedy Richard
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a655
Subject(s) - clotting time , coagulation , prothrombin time , thrombin time , activated clotting time , antithrombotic , partial thromboplastin time , bleeding time , platelet poor plasma , platelet , medicine , coagulation testing , clotting factor , chemistry , endocrinology , pharmacology , thrombin , heparin , platelet aggregation
Animal models of thrombotic and bleeding disorders are commonly used to profile the preclinical pharmacologic effects of antithrombotic drugs. This study addresses the issue of variability in the routine and some of the newer coagulation tests, such as the prothrombin time (PT), activated partial thrombplastin time (APTT), thrombin time (TT), heptest time (HT), Diluted Russell’s Viper Venom time (DRVVT) and prothrombin induced clotting time (PiCT). Citrated blood from normal healthy human volunteers (n=140), a non‐human primate colony (macaca mulatta, n = 17), male White New Zealand rabbits (n=40), Sprague Dawely rats (n=30), dogs (n=20) and pigs (n=20) (fasting state) was collected in 3.8% sodium citrate tubes in a ratio of 1:10. This blood was centrifuged at 2500 g and the plasma was then separated and used for the clotting assays. Various coagulation parameters were measured using standard reagents and instruments. The PT values ranged from 5.5 to 13.9 seconds suggesting that the response of tissue factor activation of proteases is variable. The APTT values ranged from 16.3 to 57.1 seconds. The TT values ranged from 19.8 to 38.2 seconds. Marked variations in HT, DRVVT and PiCT were also noted. These results clearly suggest that major differences in coagulation profile of animals exist which may be due to the difference in the levels of coagulation factors, activators and inhibitors.