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Platelet aggregation is not increased in db/db and ob/ob diabetic mice
Author(s) -
Davidson Lisa Beth,
Wilson Jonathan,
Maes Melissa,
McDonagh Paul F,
Coull Bruce,
Ritter Leslie
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a653-c
Subject(s) - platelet , medicine , endocrinology , diabetes mellitus , platelet aggregation , type 2 diabetes , platelet poor plasma , significant difference , chemistry , platelet rich plasma
Type 2 diabetes in humans is associated with increased platelet aggregation. The BKScg‐m+/+leprdb (db/db) and B6.V‐Lepob (ob/ob) and their respective non‐diabetic controls C57BL/6J (6J) and C57BLKS/J (BLKS), are used as models of human type 2 diabetes. The purpose of this study was to determine if platelet aggregation is increased in db/db and ob/ob mice compared to their non‐diabetic controls. Heparinized blood was drawn from 8‐16 week old male diabetic and non‐diabetic mice. Experiments were conducted using blood with either non‐normalized or normalized (3.15x105 platelets/uL +/−10%) platelet counts. Platelet aggregation, in response to 30μM ADP, was measured with a whole blood aggregometer. In non‐normalized samples, there was a significant (p<0.001) decrease in platelet aggregation between ob/ob and control mice (14+/−1.24 vs. 10+/−.55), and db/db and control mice (20.17+/−.54 vs. 13.17+/−.95 ohms). In normalized samples, there was a significant (p<0.001) decrease in aggregation between ob/ob and control mice (18+/−2.15 vs. 3.6+/−.98 ohms), but no significant difference between db/db and control mice (14.40+/−1.72 vs. 13.67+/−1.58). These results demonstrate that platelet aggregation in db/db and ob/ob mice is not increased compared to non‐diabetic controls. These findings suggest that these diabetic mouse models may not demonstrate the hypercoagulability that is observed in human type 2 diabetes. It is possible that the lack of leptin (db/db) or its receptor (ob/ob) is responsible for the responses observed. Supported by NIH NINR 05028 and NIH HLB 58859.