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Increased Nitrotyrosine Levels Observed in Acute Myocardial Infarcation. Early Diagnostic Implication
Author(s) -
Iqbal Omer,
Fareed D,
Hoppensteadt D,
Cunanan J,
Fareed J
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a651-c
Subject(s) - nitrotyrosine , cardiology , medicine , nitric oxide , nitric oxide synthase
Background Induction of endothelial nitric oxide synthase (eNOS) activity triggered by cytokines expressed as a result of inflammatory response during acute coronary syndrome is known. Nitric oxide and superoxide react in a metal‐independent manner to form peroxynitrite (ONOO‐), a potent oxidant and nitrating species that can attack a range of biologic targets. The protonated form of peroxynitrite exhibits hydroxyl‐radical like activity and carry out oxidation reactions involving 1 or 2 electron processes such as oxidation of lipids, proteins and DNA. It was hypothesized that increased nitrotyrosination occurs in acute coronary syndrome (ACS). Baseline plasma samples from a randomized controlled trial of patients with non‐ST segment elevation ACS administered with enoxaparin 1.0 mg/kg intravenously, without abciximab NICE‐1 (National Investigators Collaborating on Enoxaparin) and with abciximab (NICE‐4) were analyzed for determination of nitrotyrosine using BIOXYTECH nitrotyrosine enzyme immunoassay from OXIS Research TM (Portland, OR). Nitrotyrosine levels were significantly increased to >1500 nM (beyond the measurable range of the assay) in most patients and in others at least 2‐3 fold higher than the normal control values of 30.1±2.55 nM. These results suggest that even in the acute stage of myocardial infarction there is markedly increased nitrotyrosination.