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The Effects of Immobilized IL‐8 on Neutrophil Adhesion to ICAM‐1
Author(s) -
Lomakina Elena
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a648-a
Subject(s) - icam 1 , adhesion , phagocytosis , chemokine , chemistry , cell adhesion , biophysics , interleukin 8 , microbiology and biotechnology , adhesive , immunology , inflammation , biology , layer (electronics) , organic chemistry
The chemokine IL‐8 plays an important role in regulation of neutrophil recruitment during inflammation. In this report we examine the dynamic response of neutrophils after contacting IL‐8 immobilized on a surface. The probability of adhesion between a neutrophil and an ICAM‐1 – coated surface was determined using micropipettes to bring the cell and an ICAM‐1 coated bead into repeated contact. Cells were tested before and after interacting with a second bead coated with IL‐8 (IL‐8 ‐ fractalkine stalk chimera). In most cases the cells engulfed the IL‐8 coated bead over a period of 5 minutes at room temperature. Engulfment depended on the density of IL‐8 on the bead surface. At site densities below 200/μm 2 , approximately 15% of the beads adhered to the neutrophil but were not engulfed. Whether or not the IL‐8 coated bead was engulfed, an increase in adhesion probability to ICAM‐1 was observed to occur over a longer time scale (5–10 min). Control experiments demonstrated specificity of the response for IL‐8 as stimulus and ICAM‐1 as adhesive target. Variability in the time course of the response was observed both between cells and between donors. We hypothesize that the increase in adhesion to ICAM‐1 results from distinct signaling pathways than those leading to phagocytosis of the IL‐8 bead and can proceed independently.