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PAX 6 In A Collagen Induced Arthritis Model: Antibody ‐ Mediated Clinicopathologic Factors in Disease
Author(s) -
Prattis Susan Marie,
Kam Lily,
Asheld Wilbur,
Niwaz Tagenarayan,
Isanova Bella,
Akhtar Atrish
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a638-b
Subject(s) - collagen induced arthritis , arthritis , disease , immunology , antibody , medicine , pathology
The collagen ‐ induced model of arthritis has been developed primarily for use in examining factors affecting inflammation in rheumatoid arthritis. Using this model we are examining an additional putative pathogenic factor, PAX 6, that may affect clinicopathological expression of this disease. PAX 6, a transcription factor affecting neurodevelopment and repair following injury, is one of several classes of proteins that have been implicated in contributing to neuroinflammatory responses occuring during arthritic episodes that result in increased pain and swelling experienced by affected patients. In our study female DBA/2 mice were immunized with collagen alone, collagen and PAX 6 antibody, and saline respectively in complete freund’s adjuvant, followed by successive repeat immunizations in incomplete freund’s adjuvant. Joint swelling and modification in activity peaked by 6 weeks after the initial immunization, with PAX 6 antibody immunized animals exhibiting marked joint swelling in vivo. Hemotoxylin, eosin, and histochemical staining assessment of sampled joints suggests that arthritis was induced in our animals at variable levels in accordance with this mouse strain, and the immunogens used in this experiment. Our preliminary data suggest that incorporation of PAX 6 antibody may increase the severity of the inflammatory response. It is not yet clear whether this is specific to PAX 6 protein, or antibody, and further assays to answer this question are in progress.