Premium
The gp130 ligand Oncostatin M contributes to stem cell homing via induction of SDF‐1 in cardiac cells.
Author(s) -
Hohensinner Philipp,
Kaun Chrsitoph,
Rychli Kathrin,
Maurer Gerald,
Huber Kurt,
Wojta Johann
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a633-a
Subject(s) - oncostatin m , homing (biology) , stem cell , microbiology and biotechnology , stromal cell , biology , myocyte , glycoprotein 130 , cancer research , immunology , medicine , cytokine , signal transduction , interleukin 6 , stat3 , ecology
Stromal Derived Factor 1 (SDF‐1) is a CXC chemokine important in the homing process of stem cells to injured tissue. Novel therapies of myocardial infarction include mobilization and transplantation of stem cells, although little is known about the homing potential of the human heart. Growing evidence suggests that the glycoprotein 130 (GP‐130) receptor family is involved in survival of cardiac myocytes and in repair processes in the heart. The aim of our study was to determine if GP‐130 ligands contribute to stem cell homing via induction of SDF‐1 in cardiac cells. Results Oncostatin M (OsM) was the only GP‐130 ligand capable of inducing SDF‐1 in human adult cardiac myocytes (HACM) and human adult cardiac fibroblasts (HACF). Protein expression was up to 7 fold elevated compared to control. The induction of SDF‐1 through OsM was dose dependent. Protein data was confirmed by mRNA results. A blocker directed against p38 was able to abolish OsM induced secretion of SDF‐1 protein. Conclusion Through phosphorylation of p38 OsM induces SDF‐1 protein secretion in human cardiac cells. Our in vitro data suggests that OsM via the induction of SDF‐1 might play a key role in repair in the human heart and if also operative in vivo might be involved in homing of stem cells to this organ.