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Angiogenic peripheral blood‐derived adult stem cells
Author(s) -
Price Elmer M,
Prather Randall S,
Samuel Melissa S,
Tanner Miles A,
Latcham Shena L
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a633
Subject(s) - progenitor cell , microbiology and biotechnology , biology , vasculogenesis , endothelial stem cell , stem cell , angiogenesis , lineage markers , multipotent stem cell , mural cell , von willebrand factor , immunology , in vitro , platelet , cancer research , biochemistry
We have isolated several clonal lines of angiogenic self‐renewing progenitor cells from the blood of adult green fluorescent protein transgenic swine. The cells are designated “peripheral blood derived multipotent adult progenitor cells” (PBD‐MAPCs). These cells grow as spheroids and readily proliferate under conditions chosen to promote self‐renewal without differentiation. We have isolated 5 independent lines of these cells from 2 different animals. When plated under angiogenic differentiation conditions PBD‐MAPCs rapidly differentiate into cells that biochemically, immunologically and morphologically resemble either smooth muscle cells or endothelial cells, depending upon the differentiation conditions. When grown on collagen and fed a media that is typically used to grow vascular smooth muscle (VSM) cells, PBD‐MAPCs differentiate into cells that express α‐actin and are morphologically indistinguishable from bona fide VSM cells. Alternatively, cells differentiated on fibronectin and fed endothelial‐specific media appear cobblestone in appearance and do not express α‐actin but are positive for von Willebrand factor (vWF) and diI‐Ac‐LDL uptake, indicative of an endothelial phenotype. Finally, PBD‐MAPCs seeded on Matrigel TM and fed angiogenic media form three‐dimensional tube structures that are lined with vWF + cells. We conclude PBD‐MAPCs can differentiate into angiogenic cells that may prove useful in cell therapies directed at vascular disease. Supported by NIH PO1HL52490.