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Effect of dietary zinc on post‐absorptive activation of insulin‐ and stress‐stimulated cellular signaling using multiplex anaylsis
Author(s) -
Barnes Brian,
Gauthier Nicole A,
Scrimgeour Angus G,
McClung James P
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a626-c
Subject(s) - multiplex , insulin , microbiology and biotechnology , zinc , insulin receptor , chemistry , biology , endocrinology , bioinformatics , insulin resistance , organic chemistry
Supplemental dietary zinc (Zn) has been shown to participate in regulatory processes of cellular growth; however the cellular regulatory steps are not fully understood. We investigated the effects of Zn on insulin‐ (Akt, p70s6k and GSK‐3) and stress‐ activated (p38 MAPK, ERK, p90RSK, and JNK) signaling pathways in mouse tissues utilizing a multiplex suspension assay, which allows for simultaneous detection of multiple signaling events. Briefly, mice were fed Zn‐marginal (ZM, 5 ppm) diets for 4 wks. Thereafter, mice were fasted for 12 h (F) and/or re‐fed with ZM or Zn‐supplemental (ZS, 300 ppm) diet for 3 or 6 h. Gastrocnemius (GA) muscle and liver tissues were collected at each time point and protein content and phosphorylation of the aforementioned kinases were determined. In ZS tissue, a significant (188 %, P=0.03) increase in phosphorylation of p70s6k was seen in liver at 6 h with a similar non‐significant increase (311 %; P = 0.09) observed in GA at 3 h as compared to F. Thus, Zn may potentiate the feeding induction of translation initiation. GSK‐3 phosphorylation was elevated at 3 h (~40 %; P<0.05) in liver tissue independent of Zn status and persisted (P=0.02) at 6 h in the ZS group. Increased p38 MAPK phosphorylation was also seen (153 %; P<0.03) at 6 h in ZS liver tissue. In conclusion, multiplex analysis has proven to be a powerful tool for investigating multiple signaling events simultaneously by demonstrating that Zn may potentiate cellular regulation of signaling pathways involving p70s6k in GA and liver and GSK‐3 and p38 MAPK in liver.