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Application of mucin and Caco‐2/goblet cell co‐cultures to refine the in vitro digestion/Caco‐2 cell model for iron uptake
Author(s) -
Jin Fuxia,
McAuliffe Gretchen,
Rutzke Mike,
Shuler Michael,
Welch Ross M.,
Glahn Raymond P.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a624-c
Subject(s) - mucin , ferritin , caco 2 , bioavailability , chemistry , digestion (alchemy) , goblet cell , in vitro , biochemistry , ascorbic acid , food science , biology , chromatography , epithelium , bioinformatics , genetics
The objective of this study was to determine if commercially available mucin or Caco‐2/goblet cell co‐culture can be used to replace the 15 kD molecular weight cut‐off (MWCO) dialysis membrane used in an established in vitro digestion/Caco‐2 cell culture system. Although the current model with the 15kD membrane is well validated to human studies, use of mucin or Caco‐2/goblet cell co‐culture may make the system more physiological and enable more complete assessment of iron bioavailability from large molecular weight forms of Fe such as heme and ferritin Fe. A range of foods or Fe (i.e. FeCl 3 ± ascorbic acid (AA), cooked beef, red bean, white bean, soybean, horse spleen ferritin (HSF) and plant‐type ferritin) were subjected to in vitro digestion. In the presence of mucin, significantly more Fe was taken up from the heme Fe (86%) and ferritin (91%) samples and significantly less Fe was taken up from the white bean samples (~70%) relative to the 15kD membrane. Use of Caco‐2/goblet cell co‐culture resulted in a ~20% increase in Fe uptake from HSF and red beans and a decrease in Fe uptake from HSF + AA (~45%) and FeCl 3 + AA (73%) relative to the Caco‐2 cell system with 15kD membrane. The results indicate that the form of iron interacts with mucin and the mucus layer and has a significant effect on Fe uptake. Further refinement and characterization of the mucin and co‐culture method is needed before it can be deemed a suitable replacement for the dialysis membrane.