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Human gastric cancer‐derived endothelial cells‐specific gene expressions identified by the DNA oligomer chips
Author(s) -
Lee You Mie,
Seo Jiheun,
Kim Jin,
Kim Min A,
Kim Woo Ho
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a62-c
Subject(s) - metastasis , microbiology and biotechnology , cancer , cancer research , gene , angiogenesis , biology , thrombospondin 1 , cancer cell , tumor progression , cell adhesion molecule , chemistry , biochemistry , genetics
Angiogenesis is essential for the outgrowth and metastasis of tumors. The structure and characteristics of tumor vasculatures are known to be different from those of normal vessels. Gastric cancer is commonly known to be not‐angiogenic, but advanced gastric cancer (AGC) is very aggressive and easy to metastasize. So we would like to investigate differentially expressed genes in the endothelial cells (EC) isolated from gastric cancer compared to the normal ones. Here we purely isolated tumor and normal ECs from the AGC and normal mucosa tissues, respectively, by magnetic bead isolation techniques and identified differentially expressed genes between them using Affymetrix oligomer chip containing human whole genome. More than 3‐fold increased or decreased genes in tumor ECs compared to normal ECs were selected and analyzed within p≤ 0.001 significance. Many cytoskelecton‐related genes were up regulated, such as alpha‐actinin , myosin light peptide , LIM protein, MFAP and fillamin A . And adhesion molecules, such as, collagen type V α 2 , α 6 , III, VIII, VCAM , were highly enhanced in tumor endothelium. More dramatic different genes were protocadherin , fibrillin , IGF‐BP5 , neuropilin ‐ 2 , P1H12 (≥20 fold), suggesting those can be gastric cancer EC‐specific markers. Tumor suppressive molecules, such as interferon gamma , IAP , FEM‐1 , thrombospondin‐2 and RECK were downregulated. In addition, apoptosis‐inducing and differentiation‐inducing genes were also decreased, indicating more resistant to apoptosis and immature vessel formation in tumor. We confirmed these data with semi‐quantitative RT‐PCR and IHC.