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Antioxidant components of human milk
Author(s) -
Friel James Kenneth,
Tsopmo Apollinaire,
Kitts David,
Suh Miyoung
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a617-a
Subject(s) - antioxidant , chemistry , oxygen radical absorbance capacity , breast milk , fractionation , chromatography , high performance liquid chromatography , vitamin e , enzyme , food science , vitamin c , biochemistry , antioxidant capacity
Human milk (HM) provided better antioxidant properties than infant formulas (F) on two assays (electron spin resonance, biological oxygen monitor) before and after deactivation of the enzymes SOD, CAT, and GPx (Friel et al Pediatr Res 2002: 51 ). F doesn’t contain these enzymes but are heavily fortified with the antioxidant molecules vitamin A, C, and E. Our hypothesis: HM has antioxidant components different from those vitamins and enzymes. Extracts of HM and F were analysed by HPLC using the total UV‐Vis (210–400 nm) absorbance.There are significant differences between chromatograms. GC analysis of HM and F showed different fatty acid content including: C14:1; C20:2; C20:3n‐6; C22:1; C22:5n‐3. We are currently performing bioassay‐guided fractionation and gel permeation of the HM extract to identify and characterize the novel antioxidant components with the aim of enhancing infant formulas for those mothers who chose not to breast feed. Supported by MICH and CIHR.