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Genistein Stimulates Differentiation in Epidermal Keratinocyte through Activation of PPARs
Author(s) -
Kim Sujong,
Lee KiHwan,
Hong Il,
Hwang Jung Sun,
Rho Ho Sik,
Kim Duck Hee,
Chang Ihseop,
Lee MiOck,
Lee YongSung,
Hwang Jae Sung
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a612-a
Subject(s) - genistein , keratinocyte , hacat , biology , microbiology and biotechnology , cellular differentiation , cell culture , gene , biochemistry , endocrinology , genetics
We reported previously that genistein enhances the expression of genes involved in fatty acid catabolism through activation of peroxisome proliferator‐activated receptor (PPAR)α in HepG2 cells, and showed the beneficial effects of genistein supplementation in preventing the development of obesity in vivo. Since PPARs play important roles in skin barrier homeostasis, regulating epidermal cell growth and terminal differentiation, we examined the regulatory roles of genistein in normal human epidermal keratinocytes (NHEKs). Genistein increased the production of cornified envelope in NHEKs. Epidermal differentiation marker proteins were also increased, when examined by western blotting analysis. BrdU incorporation assay showed that genistein inhibited DNA synthesis in NHEKs in dose‐dependent manner. Next, we performed cDNA microarray analysis to identify genes that are differentially expressed during genistein‐induced differentiation of HaCaT, immortalized human keratinocyte. Taken together, this study provides a picture of the regulatory action of genistein, as an activator of PPARs in keratinocyte differentiation. This approach toward characterization of differentially expressed genes identified a number of molecules not previously known to be regulated during keratinocyte differentiation.