Premium
Cottonseed oil diets reduce human prostate LNCaP tumor growth and modulate gene expression in BALB/c nude mice
Author(s) -
IMRHAN VICKY,
Basu Arpita,
Sundaresan Sinju,
Aredo Bogale,
Bennett Michael,
Radcliffe John D
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a611-b
Subject(s) - lncap , tramp , prostate cancer , gene expression , andrology , prostate , apoptosis , matrigel , endocrinology , chemistry , medicine , cancer research , biology , gene , cancer , angiogenesis , biochemistry
Although cottonseed oil (CSO) is used widely in the food industry, very little health‐related research has been conducted. As we have previously reported, BALB/c nude mice were randomly assigned to two groups (n=11/group). They were subcutaneously inoculated with 1×10 6 LNCaP cells/0.2 mL Matrigel, and fed diets containing 20% CSO or soybean oil (SO). The CSO group had lower tumor volumes than those consuming similar levels of SO. Our current study compared the effects of high‐fat diets containing CSO or SO, on prostate specific antigen (PSA), androgen receptor (AR), transforming growth factor‐β (TGF‐β), and transitional epithelial response gene 1 (TERE1) gene expressions in tumors of the mice. By reverse transcription and relative quantification using real‐time polymerase chain reaction, tumor PSA, AR, and TGF‐β gene expressions were significantly higher in SO than CSO group (p<0.05). There was no difference in TERE1 gene expression between the two groups (p>0.05). Serum PSA levels were lower in CSO (54.5±11.4 ng/mL) than SO (112.9±9.2 ng/mL, p<0.05). This study supports our previous findings that dietary CSO reduced prostate cancer in BALB/c nude mice by modulating PSA, AR and TGF‐β gene expressions. Supported by a grant from The Texas Food and Fibers Commission.