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In Vitro Fermentation Characteristics of Oligosaccharides and Beet Pulp by Canine Fecal Bacteria
Author(s) -
Truong My H,
Hussein Hussein S.,
Boileau Thomas W.M.,
Sunvold Gregory D.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a600-a
Subject(s) - fermentation , beet pulp , dry matter , pulp (tooth) , food science , chemistry , prebiotic , incubation , feces , completely randomized design , zoology , microbiology and biotechnology , biology , biochemistry , medicine , pathology
The objective was to determine the prebiotic potential of three oligosaccharides (fructooligosaccharides [FOS], galactooligosaccharides [GOS], and lactosucrose) in supporting canine colon health. To achieve that, 240 serum bottles (125 mL each; 192 for the test substrates and 48 for blanks) were used in an in vitro fermentation experiment. The experimental design was a completely randomized design with treatments being arranged as a 4 × 8 factorial. The main factors were four substrates (beet pulp [a standard fiber source in premium canine diets], FOS, GOS, and lactosucrose) and eight incubation times (3, 6, 9, 12, 15, 18, 21, and 24 h). Three fecal inocula were prepared from three healthy dogs and were used to inoculate the bottles with (0.3 g dry matter each) or without (blanks) substrates. Each bottle contained 27 mL of an anaerobic medium (Sunvold et al., J. Anim. Sci. 73:3639–3648), was inoculated with 3 mL of the designated inoculum, and was incubated at 39°C. Across incubation times, the extent of dry matter disappearance and total gas production were higher (P < 0.05) for the three oligosaccharides (averaging 99.1% and 66.7 mL, respectively) than for beet pulp (33.8% and 33.1 mL, respectively). Lactate concentrations were 18.1, 142.5, 62.8, and 112.3 μg/mL for beet pulp, FOS, GOS, and lactosucrose, respectively. The results suggest FOS to have the highest potential to increase lactate‐producing bacteria in the canine colon.