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Caloric intake and dietary fat to carbohydrate ratio influence body weight accretion and adiposity in rats fed by total enteral nutrition
Author(s) -
Shankar Kartik,
Ferguson Matthew E,
Dallari Tammy M,
Ronis Martin J J,
Badger Thomas M
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a581-c
Subject(s) - calorie , carbohydrate , endocrinology , medicine , chemistry , weight loss , weight gain , obesity , body weight
Consumption of high energy high‐fat diets leads to weight gain and adiposity in rodents. However the relative roles of higher caloric intake vs. increased dietary fat remain unclear. Low carbohydrate high fat diets, like the Atkins diet, claim to promote weight loss. We employed a total enteral nutrition model to evaluate roles of caloric intake and dietary fat to carbohydrate ratio in mediating weight gain. Female Sprague‐Dawley rats were fed either 187 kcal/kg 3/4 /d (normal calorie, NC, NRC requirements) or 220 kcal/kg 3/4 /d (overfed 15% excess calories/d, high calorie, HC) via intragastric infusion. NC and HC diets were either low fat (LF, 5% calories from fat, corn oil) or high fat (HF, 45% calories from fat, ~20‐fold higher fat/carbohydrate than LF). LF and HF diets were isocaloric. Protein (casein) was 20% of caloric intake and carbohydrate calories contributed to 75% and 35% in the LF and HF diets, respectively. A separate group of rats were fed regular rodent chow (ad libitum). After 6 weeks of infusion of diets, body weights (BW) of rats fed NC‐HF matched ad lib fed controls. HC‐HF rats showed significantly higher (152%) BW compared to NC‐HF and ad lib fed controls. However, both NC and HC rats fed LF diets consistently showed ~20% higher BW compared to rats fed HF diets. Consistent with BW data, body composition as assessed by Echo MRI revealed higher fat mass/BW in rats fed HC diets. Greater fat mass/BW was also observed in NC‐LF rats as compared to NC‐HF fed rats. Analysis of oral glucose tolerance tests to evaluate insulin resistance as a function of caloric and diet fat intake is ongoing. Supported in part by ARS CRIS #6251‐51000‐003‐065