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Media phosphate and iron(II) supplementation affect Penicillium cell wall polymer structure
Author(s) -
Alvis Alan J.,
Carsella James S.,
Bonetti Sandra J.,
Lehmpuhl David W.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a58-a
Subject(s) - phosphate , mycelium , chemistry , ferrous , cell wall , polymer , biochemistry , nuclear chemistry , food science , botany , organic chemistry , biology
Our investigations focus on the effect of media nutrients, specifically phosphate and iron(II) on the composition of Penicillium fellutanum cell wall‐derived polymers. In these studies we use four different media: high phosphate standard growth (HPSG), high phosphate high iron standard growth (HPSG Fe), low phosphate standard growth (LPSG), and low phosphate high iron standard growth (LPSG Fe). Mycelia obtained from these media are autoclaved in water to obtain heat‐solubilized, cell wall‐derived glycopeptides. Structures of heat‐solubilized large (>14 kD) and small (<14 kD) mycelial glycopeptides have been characterized by GC/MS, NMR, and carbohydrate, phosphate, and protein analyses. We report that the phosphodiester composition in these polymers is a function of media phosphate and ferrous ion. Proton‐decoupled 13 C NMR spectra indicate that the large autoclaved mycelial polymers or LAMs resemble the 65 kD extracellular peptidophosphogalactomannans (pPGMs) in structure, however, they are very different from the small mycelial polymers or SAMs that consist primarily of glucose and peptides. Supported by NIH/NIGMS MBRS Grant# 2S06 GM008197.